The soul, in many mythological, religious, philosophical, and psychological traditions, is the incorporeal and, in many conceptions, immortal essence of a person, living thing, or object.
Some enemies Daniel encounters are soulless, however, and killing them does not fill the Chalice.
There are also characters with good souls. Killing such characters drains the Chalice of its power instead.
Hypnotized citizens of a once thriving town.
They'll happily invite you in for tea, but if they ask to borrow a shovel, you should probably decline.
Are these good men? Their farmyard toil puts food on the plates of the nation, but at what price? Could mutant pumpkins be the result of forcing innocent pumpkins to consume their fleshy brethren? Does safeguarding one's crops justify deployment of homicidal scarecrows? Who will speak for the wolves?
Save them - or don't - it's up to you.